SPUTUM AFB DSSM >

• Sputum Acid Fast Bacilli
• Direct sputum smear microscopy

SPUTUM COLLECTION                                                       
The most productive sputum can be collected in the morning.                                                                            
1. Expectorated Sputum
a. Rinse the mouth and gargle with water. Do not brush teeth prior to collection.
b. Take a deep breath and make a deep cough. Good quality sputum should not contain saliva. Specimen should be collected in a sterile leak-proof container and delivered to the laboratory without delay.
c. Properly close and wipe dry the container. Label sample container with patient's complete name, date and time of collection.

2. Induced Sputum
a. Rinse the mouth and gargle with water. Do not brush teeth prior to collection.
b. Using a nebulizer, have the patient inhale approximately 20 to 30 mL of 3% NaCl.
c. Take a deep breath and make a deep cough. Collect the induced sputum into a sterile leak-proof container delivered to the laboratory without delay.
d. Properly close and wipe dry the container. Label sample container with patient's complete name, date and time of collection.

It is used to detect Mycobacterium tuberculosis in sputum samples to confirm or rule out a diagnosis of tuberculosis in patients.

1 - 3 ML

Sputum

NEGATIVE